Chloride Inhibition of Spinach Nitrate Reductase
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چکیده
منابع مشابه
Analysis of nitrate reductase mRNA expression and nitrate reductase activity in response to nitrogen supply
Nitrate is one of the major sources of nitrogen for the growth of plants. It is taken up by plant roots and transported to the leaves where it is reduced to nitrite in the. The main objective of this research was to investigate stimulatory effects of sodium nitrate, potassium nitrate, ammonia and urea on the production/generation of the nitrate reductase mRNA in Triticum aestivum plants. The pl...
متن کاملStudies by electron-paramagnetic-resonance spectroscopy of the molybdenum centre of spinach (Spinacia oleracea) nitrate reductase.
The molybdenum centre of spinach (Spinacia oleracea) nitrate reductase has been investigated by e.p.r. spectroscopy of molybdenum(V) in reduced forms of the enzyme. The resting enzyme gives no signals attributable to Mo(V). However, on reduction with NADH, Mo(V) signals appeared at relatively short reaction times but decreased again on prolonged exposure to excess of the substrate as the enzyme...
متن کاملInhibition of the nitrate reductase complex by dibromothymoquinone.
The plastoquinone antagonist 2,5-dibromothymoquinone was found to inhibit NO-3 reduction from NADH by the nitrate reductase complex from wheat. It accepts electrons from NADH through the NADH dehydrogenase activity of the nitrate reductase. However, it does not inhibit the reduction of 2,6-dichlorophenol-indophenol by the enzyme. This suggests that the two compounds may be accepting electrons a...
متن کاملReconstitution in vitro of nitrate reductase from apoprotein of molybdenum-deficient spinach.
Hirasawa, E. & Suzuki, Y. (1975) Phytochemistry 14,99-101 McGowan, R. E. & Muir, R. M. (1971) Plant Physiol. 47, 644-648 Smith, T. A. (1974) Phytochemiktry 13,1075-1081 Smith, T. A. (1975) Phytochemistry 14,865-890 Smith, T. A. & Bickley, D. A. (1974) Phytochemistry 13, 2437-2443 Tabor, C. W. & Kellogg, P. D. (1970) J. Biol. Chem. 245, 54245433 Weaver, R. H. & Herbst, E. J. (1958) J. Biol. Chem...
متن کاملIdentification of Ser-543 as the major regulatory phosphorylation site in spinach leaf nitrate reductase.
Spinach leaf NADH:nitrate reductase (NR) responds to light/dark signals and photosynthetic activity in part as a result of rapid regulation by reversible protein phosphorylation. We have identified the major regulatory phosphorylation site as Ser-543, which is located in the hinge 1 region connecting the cytochrome b domain with the molybdenum-pterin cofactor binding domain of NR, using recombi...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1989
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.90.1.70